Coding

Part:BBa_J40004:Design

Designed by: Jay Nadeau   Group: iGEM06_McGill   (2006-10-26)


Jun-BetaE-YFPN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 82
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 1438
    Illegal AgeI site found at 1213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contained an EcoRI site that was removed by converting it to a Mun I site using oligo-based mutagenesis. Three Pst I sites are still found within the gene and will be removed at a later date by site-specific mutagenesis.


Source

Jun-IgA beta provided by LA Fernandez, Department of Microbial Biotechnology, Madrid. The YFP N-terminus was inserted into this plasmid as a PCR fragment; sequence determined by Hu and Kerppola, Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol. 2003 May;21(5):539-45

References